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gdf11 1e6  (Novus Biologicals)


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    Structured Review

    Novus Biologicals gdf11 1e6
    Gdf11 1e6, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdf11+1e6/GDF-11%2FBMP-11+Antibody+(1E6)/pmc05726799-1308-1-2
    Average 88 stars, based on 1 article reviews
    gdf11 1e6 - by Bioz Stars, 2026-10
    88/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Tumor Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer
    Article Snippet: Slides were washed with PBS and blocked with 1× Western Blocking reagent (Roche) in PBS + 0.3% Tween-20 (frozen sections) or 1× Western Blocking reagent (Roche) in PBS + 0.1% Tween-20 (paraffin sections). .. The following primary antibodies were diluted into blocking buffer at the indicated dilutions and incubated overnight at room temperature: GDF11–EPR (Abcam #ab124721, 1:200), GDF11–1E6 (Novus Biological #H00010220-M03, 1:200–1:60 depending on the lot of antibody), GDF11 (R&D Systems #MAB19581, 1:200), GM130 (BD Biosciences #610823, 1:200), ID2 (Sigma #HPA027612, 1:200). ..

    Incubation:

    Article Title: Tumor Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer
    Article Snippet: Slides were washed with PBS and blocked with 1× Western Blocking reagent (Roche) in PBS + 0.3% Tween-20 (frozen sections) or 1× Western Blocking reagent (Roche) in PBS + 0.1% Tween-20 (paraffin sections). .. The following primary antibodies were diluted into blocking buffer at the indicated dilutions and incubated overnight at room temperature: GDF11–EPR (Abcam #ab124721, 1:200), GDF11–1E6 (Novus Biological #H00010220-M03, 1:200–1:60 depending on the lot of antibody), GDF11 (R&D Systems #MAB19581, 1:200), GM130 (BD Biosciences #610823, 1:200), ID2 (Sigma #HPA027612, 1:200). ..

    Staining:

    Article Title: Tumor Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer
    Article Snippet: Antigen-retrieved tissue was incubated with one of the following primary antibodies for 30 min at room temperature: GDF11–EPR (Abcam #ab124721, 1:400), Ki67 (Abcam #ab16667, 1:200), cleaved caspase-3 (Cell Signaling #9661, 1:500), and E-cadherin (Sigma #HPA004812, 1:500). .. For GDF11–1E6 (Novus Biological #H00010220-M03), there was considerable lot-to-lot variation, which required a range of staining conditions optimized for each lot: no antigen retrieval (1:100 antibody for 30 min at room temperature) or low-pH EnVision FLEX Target Retrieval Solution for 20 min at 97°C (1:60 – 1:300 antibody for 60 min at room temperature). ..



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    Novus Biologicals gdf11 1e6
    Gdf11 1e6, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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